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. 2017 Sep 5;7:10438. doi: 10.1038/s41598-017-10776-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kinetics of disease in C57BL/6 mice infected with the N67C parasite. (a) Parasitemia, (b) survival, (c) body weight, (d) hematocrit, (e) number of splenic leukocytes, and (f) total spleen weight over the course of N67C infection. (g) Representative photomicrographs of hematoxylin and eosin (H&E) stained spleen sections from non-infected (NI) and N67C-infected mice at days 4 and 6 post-infection (dpi) showing progressive loss of cellularity mainly in the red pulp and marginal zone (40X magnification). The results are expressed as mean ± SEM (3–5 mice) and are representative of two or three independent experiments. Kruskal-Wallis test, *p < 0.05, **p < 0.01, and ***p < 0.001 (compared to NI group).