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. 2017 Sep 5;7:10438. doi: 10.1038/s41598-017-10776-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Retention of macrophages (Mɸ) subtypes and monocytes in IFN-γ^−/− mice. (a) Representative flow cytometry plots of red pulp Mɸ (F4/80⁺ CD11b^-) of WT, IFN-γ^−/− and anti-IFN-γ mAb treated mice day 4 p.i. (b,c) Percentage and absolute number of red pulp Mɸ. (d) Representative flow cytometry plots of inflammatory monocytes (CD11b⁺ CD115⁺). (e,f) Percentage and absolute number of inflammatory monocytes. Data in (b,c and e,f) are mean ± SEM (3–5 mice) and are representative of two experiments; Kruskal-Wallis test, *p < 0.05, and ***p < 0.01. (g) Immunofluorescent staining of splenic Mɸ sub-populations using specific markers as described in⁴⁰. The red pulp Mɸ was stained by F4/80 expression, marginal zone Mɸ by CD209b, and metallophilic Mɸ by CD169 (40X magnification). The arrowheads point to stained cells of different sub-populations of macrophages.