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. 2017 Sep 5;7:10438. doi: 10.1038/s41598-017-10776-2

Figure 7.

Figure 7

Lymphocyte and IFN-γ mediated apoptosis as one of the major pathways of splenic cell death. Total spleen tissue homogenates were analyzed by Western blot using antibodies as indicated. (a) Detection of cleaved caspase-3, cleaved caspase-8, cleaved PARP, and granzyme B (GrzB) in spleen tissue homogenates of WT naïve (NI) and N67C infected mice at days 2, 4, and 6 p.i. (b) Immunostaining of spleen sections for cleaved caspase-3. (c) Detection of cleaved caspase-3, cleaved caspase-8, cleaved PARP, and granzyme B at day 4 p.i. in spleen tissues of WT and RAG2−/− mice. (d) Detection of cleaved caspase-3, cleaved caspase-8, cleaved PARP, and granzyme B in the spleen tissues of WT, IFN-γ−/− or mice treated with anti-IFN-γ mAb. (e) Signals of cleaved caspase-3 in bone marrow (BM) of naïve (NI) or infected WT and IFN-γ−/− mice. (f) Total cell numbers from BM femurs of WT and IFN-γ−/− mice. Data are mean ± SEM (3–5 mice) and are representative of two independent experiments. (g–j) Host survival (g), parasitemia (h), body weight (i), and representative photomicrographs of hematoxylin and eosin (H&E) stained spleen sections (j) from N67C-infected WT and FPR1−/− mice (40X magnification). Kruskal-Wallis test, *p < 0.05, ***p < 0.001. Gels for a, c, d, and e, were run under the same experimental conditions, and the figures were cropped from original gel images. The original Western blot images can be found in Supplementary Fig. 5.