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. 2017 Sep 5;7:10417. doi: 10.1038/s41598-017-11021-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mean proportional survival of 1 and 4-week-old flies after cuticle inoculation or haemocoelic injection with Beauveria bassiana spores. To make both infection treatments comparable, we selected data from the timepoint where overall mean proportional survival was closest to 50% for each treatment route: day 7 for cuticle inoculation and day 5 for haemocoelic injection (see methods). (A) Female flies: both infection treatments showed the same age-dependent decline in survivorship. (B) male flies: cuticle inoculated flies showed a strong decrease in survival while aging, whereas pathogen injected flies showed only a minimal change in survivorship. Uninfected control flies showed minimal mortality during this time frame (see text). Error bars show ± SE; significance of comparisons shown by ***P < 0.001.