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. 2017 Sep 5;7:10416. doi: 10.1038/s41598-017-10966-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of different promoter on transfection efficiency and transient transgene expression. The pIRES-mediated vectors containing CMV, CAG, CHEF-1α, CMV mutant, HEF1-α, mouse CMV, CAG and PGK were transfected into CHO cells, and CHO cells were cultured in absence of G418 selection pressure for 48 h. (A) The eGFP of cells fluorescence profile was observed under fluorescence microscope; (B) The transfection efficiency were obtained using eGFP antibody analysis. (C) eGFP proteins transient expression levels. CMV promoter was regarded as 100, the MFI of other promoter were calculated. CMV, Cytomegalovirus major immediate-early; CAG, the CMV enhancer fused to the chicken beta-actin promoter; CHEF-1α, Chinese hamster elongation factor-1α; mouse CMV, mouse cytomegalovirus; HEF-1α, human elongation factor-1α; PGK, phosphoglycerate kinase; and CMV protein mutant, CAG enhancer. EPO, erythropoietin; SpA, simian virus 40 early polyadenylation signal; eGFP, enhanced green fluorescence protein.