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. 2017 Sep 5;7:10416. doi: 10.1038/s41598-017-10966-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cells were collected at 10 generation post-transfection and the eGFP MFI was measured by flow cytometry. (A) The stably transfected cells were screened in medium containing G418 (800 μg/mL). The eGFP MFI of stably transfected cell lines containing different promoters were detected. Black bar represent the results from after 10 generations analyzed by flow cytometry. (B) Fold statistical analysis results of expression level, and the eGFP MFI was normalized to CMV promoter. Three stably transfected pools were generated for each vector. Cells were collected and measured for the eGFP MFI with the FACS Calibur (*P < 0.05, **P < 0.01).