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. 2017 Sep 5;7:10416. doi: 10.1038/s41598-017-10966-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of EPO protein. The vectors containing EPO were transfected into CHO cells, and the stably transfected cells were screened under G418 selective pressure and thirty single cell clones were picked out. Cells supernatant were collected for analysis by ELISA to determine volumetric EPO production (mg/L). (A) CHEF-1α-EPO-containinng vector; (B) CMV-EPO-containing vector; (C) Western blot analysis. Lane 1, Un-transfected cells; Lane 2, CMV promoter; 3, CHEF1-α promoter, 4, Positive control.