Table 2.
The designed multigroup PCR primers.
| Primer ID | Genus name | Primer sequence (5′ - 3′) | T m (°C) | Amplicon length (bp) |
|---|---|---|---|---|
| ActF | Actinomyces | Pa-Eb-GCGAAGAACCTTACCAAGGC | 56.2 | 142 |
| ActR | Actinomyces | Qc-E-TGACGACAACCATGCACCAC | ||
| PreF | Prevotella | P-E-GAACCTTACCCGGGCTTGAA | 54.2 | 138 |
| PreR | Prevotella | Q-E-TGACGACAACCATGCAGCAC | ||
| StrF | Streptococcus | P-E-AACGATAGCTAATACCGCAT | 46.1 | 139 |
| StrR | Streptococcus | Q-E-TAATACAACGCAGGTCCATC | ||
| TreF | Treponema | P-E-CGCGAGGAACCTTACCTGGG | 53.7 | 137 |
| TreR | Treponema | Q-E-ACGACAGCCATGCAGCACCT | ||
| LepF | Leptotrichia | P-E-ACGCGAGGAACCTTACCAGA | 53.5 | 139 |
| LepR | Leptotrichia | Q-E-CAGCCATGCACCACCTGTCT | ||
| NeiF | Neisseria | P-E-CGGGTTGTAAAGGACTTTTG | 49.2 | 135 |
| NeiR | Neisseria | Q-E-AGTTAGCCGGTGCTTATTCT | ||
| SelF | Selenomonas | P-E-CTTCGGATCGTAAAGCTCTG | 51.4 | 142 |
| SelR | Selenomonas | Q-E-TAGTTAGCCGTGGCTTCCTC | ||
| CapF | Capnocytophaga | P-E-TACGCGAGGAACCTTACCAA | 51.3 | 138 |
| CapR | Capnocytophaga | Q-E-ACAACCATGCAGCACCTTGA | ||
| PorF | Porphyromonas | P-E-CAGCCAAGTCGCGTGAAGGA | 54.0 | 172 |
| PorR | Porphyromonas | Q-E-CTGGCACGGAGTTAGCCGAT | ||
| FusF | Fusobacterium | P-E-ACGCGTAAAGAACTTGCCTC | 51.4 | 75 |
| FusR | Fusobacterium | Q-E-ACGCGTAAAGAACTTGCCTC | ||
| MycF | Mycoplasma | P-E-GATGGAGCGACACAGCGTGC | 55.2 | 187 |
| MycR | Mycoplasma | Q-E-GCGGCTGCTGGCACATAGTT | ||
| DiaF | Dialister | P-E-CGGAATTATTGGGCGTAAAG | 53.1 | 161 |
| DiaR | Dialister | Q-E-CTTTCCTCTCCGATACTCCA | ||
| EubF | Eubacterium | P-E-GATACCCTGGTAGTCCACGC | 51.6 | 146 |
| EubR | Eubacterium | Q-E-CTCCCCAGGTGGAATACTTA | ||
| FirF | Firmicutes | P-E-GATACCCTGGTAGTCCACGC | 51.6 | 146 |
| FirR | Firmicutes | Q-E-CTCCCCAGGTGGAATACTTA | ||
| PepsF | Peptostreptococcus | P-E-TAATTCGAAGCAACGCGAAG | 52.7 | 154 |
| PepsR | Peptostreptococcus | Q-E-CGACAACCATGCACCACCTG | ||
| KinF | Kingella | P-E-GGAATTACTGGGCGTAAAGC | 52.4 | 168 |
| KinR | Kingella | Q-E-AATTCTACCCCCCTCTGACA | ||
| PepnF | Peptoniphilus | P-E-ATCACTGGGCGTAAAGGGTT | 51.1 | 186 |
| PepnR | Peptoniphilus | Q-E-CGCATTTCACCGCTACACTA | ||
| LachF | Lachnospiraceae | P-E-CAACGCGAAGAACCTTACCA | 54.2 | 146 |
| LachR | Lachnospiraceae | Q-E-GACGACAACCATGCACCACC | ||
| PasF | Aggregatibacter b | P-E-CGGGTTGTAAAGTTCTTTCG | 48.4 | 135 |
| PasR | Aggregatibacter | Q-E-TTAGCCGGTGCTTCTTCTGT | ||
| PasF | Haemophilus | P-E-CGGGTTGTAAAGTTCTTTCG | 48.4 | 135 |
| PasR | Haemophilus | Q-E-TTAGCCGGTGCTTCTTCTGT | ||
| LactF | Lactobacillus | P-E-CCGCAACGAGCGCAACCCTT | 55.3 | 103 |
| LactR | Lactobacillus | CCTCCGGTTTGTCACCGGCA | ||
| TM7F | TM7 | P-E-GGGCGTAAAGAGTTGCGTAG | 49.2 | 185 |
| TM7R | TM7 | Q-E-TACGGATTTCACTCCTACAC | ||
| GemF | Gemella | AAAGCTCTGTTGTTAGGGAA | 46.8 | 98 |
| GemR | Gemella | P-E-GGTGGCTTTCTGGTTAGGTA | ||
| PseF | Pseudomonas | GGCGGCAGGCCTAACACATG | 56.5 | 99 |
| PseR | Pseudomonas | P-E-TTACTCACCCGTCCGCCGCT | ||
| VeiF | Veillonella | P-E-GTAAAGCTCTGTTAATCGGG | 48.1 | 100 |
| VeiR | Veillonella | GTGGCTTTCTATTCCGGTAC | ||
| MogF | Mogibacterium | P-E-CACGTGCTACAATGGTCGGT | 50.7 | 101 |
| MogR | Mogibacterium | ATCCGAACTGGGATCGGTTT | ||
| CatF | Catonella | P-E-CATGCAAGTCGAACGGAGAT | 50.8 | 101 |
| CatR | Catonella | GTTACTCACCCGTCCGCCAC |
aCCAAGGCGGCCGTACG; bCTGAAG; cCCGACGTCGACTATCCAT.