Table 1.
Results of tested stationary phase.
| Column | Analyte | R s | T f | α | N |
|---|---|---|---|---|---|
| HPLC | |||||
| Hypersil ODS (250 × 4.6 mm, 5 μm) |
Vitamin D3 | — | 1.11 | — | 4132 |
| MK-7 | 13.14 | 1.36 | 5.64 | 8329 | |
| Venusil XBP C18 (250 × 4.6 mm, 5 μm) |
Vitamin D3 | — | 1.14 | — | 3763 |
| MK-7 | 12.54 | 1.31 | 5.31 | 7296 | |
| ACE 5 C18 (250 × 4.6 mm, 5 μm) |
Vitamin D3 | — | 1.11 | — | 5867 |
| MK-7 | 15.92 | 1.12 | 6.83 | 9683 | |
| Purespher® RP-18 (250 × 4.6 mm, 5 μm) |
Vitamin D3 | — | 1.37 | — | 3923 |
| MK-7 | 13.45 | 1.09 | 5.91 | 8209 | |
|
| |||||
| UHPLC | |||||
| ACE Excel 2 C18-PFP (100 × 2.1 mm, 2 μm) |
Vitamin D3 | — | 0.94 | — | 23289 |
| MK-7 | 27.61 | 0.98 | 4.61 | 28521 | |
| Waters ACQUITY 1.7 BEH C 18 (100 × 2.1 mm, 2 μm) |
Vitamin D3 | — | 1.32 | — | 19342 |
| MK-7 | 24.23 | 1.15 | 4.25 | 25029 | |
| Agilent Poroshell 2.7 120 EC C18 (100 × 2.1 mm, 2 μm) |
Vitamin D3 | — | 1.23 | — | 15687 |
| MK-7 | 21.54 | 1.15 | 3.71 | 22143 | |
| Phenomenex Kinetex 2.6 C18 (100 × 2.1 mm, 2 μm) |
Vitamin D3 | — | 1.07 | — | 11981 |
| MK-7 | 19.21 | 1.12 | 3.31 | 19814 | |