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Evidence for EsnABC association via two-hybrid analysisa
| Coexpression with pEsnA bait | LacZ activity (ΔOD420 min−1 · OD600−1) |
|---|---|
| pSR658 (negative control) | 1,378.5 ± 131.8 |
| pEsnA prey (EsnA-EsnA) | 287.9 ± 15.6 |
| pEsnB prey (EsnA-EsnB) | 473.6 ± 44.2 |
| pEsnC prey (EsnA-EsnC) | 448.3 ± 46.5 |
LacZ activity is calculated based on the results from biological triplicates ± standard deviations. Lower LacZ activity indicates stronger protein-protein associations, causing stronger repression.
