Table I.
Gene | Direction | Primer sequence (5′-3′) |
---|---|---|
Akt1 | Forward | GAAGGACGGGAGCAGGC |
Reverse | CTCACGCGCTCCTCTCAG | |
Akt2 | Forward | TGCCACCATGAATGAGGTGAA |
Reverse | GTACCCAATGAAGGAGCCGT | |
Akt3 | Forward | TTTCTCCAAGTTGGGGGCTC |
Reverse | CCCCTCTTCTGAACCCAACC | |
Bcl-2 | Forward | TTTGTGGAACTGTACGGCCC |
Reverse | GTTGACTTCACTTGTGGCCC | |
Bax | Forward | AGCAGATCATGAAGACAGGGG |
Reverse | TGCTCGATCCTGGATGAAACC | |
β-actin | Forward | CTCACCATGGATGATGATATCGC |
Reverse | AGGAATCCTTCTGACCCATGC |
Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein.