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. 2017 Jul 28;14(4):2801–2808. doi: 10.3892/etm.2017.4846

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Copyright: © Leng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Isolating ALDH^high CSCs from spheroid cells with PI staining. (A) Exclusion of dead cells by PI staining prior to flow cytometry. The mean percentages of ALDH^high cancer stem cells in DLD-S, HT29S and HCT116S cells were 0.09±0.01, 0.04±0.01, and 0.09±0.01%, respectively. (B) The dead cells as distinguished by PI staining were distributed in the ALDH^high subpopulation (Green). Consequently, the dead cells were counted as ALDH^high cells. (C) The ALDH^high cells from DLD-1, HT29 and HCT116 adherent cells were 0.01±0.01 to 0.02±0.01%, respectively. ALDH-DEAB was used as a control. All data are presented as mean ± standard deviation of three independent experiments. *P<0.05 vs. ALDH. PI, propidium iodide; ALDH^high, high aldehyde dehydrogenase; DEAB, diethylaminobenzaldehyde; SSC-A, side scatter area.