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. 2017 Aug 2;14(4):2823–2830. doi: 10.3892/etm.2017.4870

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Copyright: © Deng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effects of FOXC1 overexpression on OS cell proliferation and migration. The OS cell line U2OS was transfected with a pcDNA3.1-FOXC1 expression plasmid or with a blank vector, as a control. (A) Reverse transcription-quantitative polymerase chain reaction and (B) western blot analysis were performed to measure the expression of FOXC1 at the mRNA and protein levels. (C) MTT and (D) wound healing assays were conducted to assess the proliferation and migration of transfected cells. Magnification, ×40. **P<0.01 vs. control. FOXC1, forkhead box C1; OS, osteosarcoma.