Figure 5.

Targeting of FOXC1 by miR-133b in OS. U2OS cells were transfected with miR-133b or miR-NC. (A) RT-qPCR and (B) western blot analysis were performed to measure the expression of miR-133b and FOXC1 protein, respectively. U2OS cells were also transfected with miR-133b inhibitor or NC inhibitor. (C) RT-qPCR and (D) western blot analysis were performed to measure the expression of miR-133b and FOXC1 protein, respectively. **P<0.01 vs. NC. FOXC1, forkhead box C1; miR, microRNA; OS, osteosarcoma; NC, negative control; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.