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Effect of buffer on EDC cross-linking reaction with gelatin: pH 5, EDC at 2.5× gelatin carboxyl groups, gelatin 10 mg/mL, buffers at 0.1 M, 1 h reaction, % HMW determined as shown in Figure 1. Control is gelatin in buffer without EDC. Mean ± SD (n = 3). *p < 0.05 vs control. #p < 0.05 vs MES 1 h.
