Fig. 5.

Regulation of EMT by ERp29 was dependent on PI3K/Akt pathway. (a) GSEA plot showing that ERp29 expression negatively correlated with Akt-activated gene signatures (REACTOME_PI3K_AKT_ACTIVATION) and positively correlated with GSK3β-pathway (BIOCARTA_GSK3_PATHWAY) (left panel). Western blot analysis of total Akt, pAKT(Ser473), pAKT(Thr308), GSK-3β and pGSK3β(Ser9) expression in the indicated gastric cancer cell lines. (b) GSEA plot showing that ERp29 expression negatively correlated with Akt/mTOR pathway (MTOR_UP.N4.V1_UP, MTOR_UP.N4.V1_DN). Western blot analysis of mTOR, p-mTOR expression in indicated gastric cancer cell lines. (c) LY294002 and rapamycin reversed the effect of ERp29 overexpression on the cell migratory and invasive abilities. (d) CHIR99021 reversed the effect of ERp29 knockdown on the cell migratory and invasive abilities. *P < 0.05