Table 3.
Unaffected expression of neutrophil effectors during C. albicans infection
| Gene | Protein | Average RPKM | ± SD | Rated |
|---|---|---|---|---|
| S100A9 | S100A9 | 17,890 | 3030 | ++++ |
| S100A8 | S100A8 | 4721 | 837 | ++++ |
| NCF2 | NADPH oxidase, p67 | 669 | 103 | +++ |
| LYZ | lysozyme C | 435 | 91 | +++ |
| S100A12 | S100A12 | 372 | 78 | +++ |
| NCF4 | NADPH oxidase, p40 | 293 | 40 | +++ |
| MMP9 | gelatinase B | 258 | 49 | +++ |
| RAC2 | NADPH oxidase, Rac1 or Rac2 | 236 | 29 | +++ |
| CYBA | NADPH oxidase, p22-PHOX | 115 | 19 | ++ |
| NCF1 | NADPH oxidase, p47 | 62 | 13 | ++ |
| CAT | catalase | 51 | 8 | ++ |
| RAC1 | NADPH oxidase, Rac1 or Rac2 | 25 | 5 | + |
| CAMP | cathelicidin, LL-37 | 25 | 6 | + |
| CYBB | NADPH oxidase, gp91-PHOX | 16 | 3 | + |
| HMGB1 | high-mobility group protein 1/amphoterin | 8 | 1 | + |
| TCN1 | transcobalamin-I | 4 | 1 | – |
| CRISP3 | cysteine-rich secretory protein-3 | 1 | 0 | – |
| LTF | lactotransferrin | 1 | 0 | – |
| ELANE | neutrophil elastase | 0 | 0 | – |
| DEFA1B | defensin 1 | 0 | 0 | – |
| DEFA1 | neutrophil defensin 1 | 0 | 0 | – |
| MMP8 | neutrophil collagenase | 0 | 0 | – |
| CTSG | cathepsin G | 0 | 0 | – |
| MPO | myeloperoxidase | 0 | 0 | – |
| PRTN3 | proteinase 3 | 0 | 0 | – |
| BPI | bactericidal/permeability-increasing protein | 0 | 0 | – |
| DEFA3 | neutrophil defensin 3 | 0 | 0 | – |
| DEFA4 | neutrophil defensin 4 | 0 | 0 | – |
| DEFA5 | defensin 5 | 0 | 0 | – |
| DEFA6 | defensin 6 | 0 | 0 | – |
The transcript levels of typical neutrophil antimicrobial effector genes that were not differentially expressed during cocultivation with C. albicans was determined over all time points and conditions. Average and SD RPKM values are displayed and rated to illustrate the expression level. ++++: RPKM >1000; +++: RPKM ≥200; ++: RPKM ≥30; +: RPKM ≥5; −: RPKM ≥0