Table 1.
Effect of mesenchymal stem cells on wild-type and CXCR3–/– fibroblast expression of genes encoding extracellular matrix molecules
| Gene symbol | GenBank™ accession number | Common name | Fold change + MSC/control (WT) | p value | Fold change + MSC/control (KO) | p value |
|---|---|---|---|---|---|---|
| Fn1 | NC_000067 | Fibronectin 1 | 1.91 | 0.0524 | 3.21 | 0.0317 |
| Tnc | NC_000070 | Tenascin C | 1.33 | 0.0989 | 2.36 | 0.0497 |
| Lama1 | NC_000083 | Laminin, alpha 1 | 0.89 | 0.5424 | 1.34 | 0.0605 |
| Col3a1 | NC_000067 | Procollagen, type III, alpha 1 | 2.67 | 0.04 | 2.85 | 0.035 |
| Actg2 | NC_000072 | Actin, gamma 2, smooth muscle, enteric | 0.25 | 0.1732 | 1.77 | 0.0739 |
| Col4a3 | NC_000067 | Procollagen, type IV, alpha 3 | 2.63 | 0.0352 | 3.09 | 0.0204 |
| Mmp9 | NC_000068 | Matrix metallopeptidase 9 | 2.41 | 0.0052 | 0.88 | 0.605 |
| Tgfb-1 | NC_000073 | Transforming growth factor beta-1 | 0.64 | 0.1862 | 0.7 | 0.1773 |
| Gapdh | NC_000072 | Glyceraldehyde-3-phosphate dehydrogenase | 1.6 | 0.0089 | 1.98 | 0.0056 |
RNA was extracted from dermal fibroblasts cocultured with MSCs for 36 hours. Gene expression levels were measured. Fold changes in mRNA levels were determined by dividing gene expression levels of fibroblasts cocultured with mesenchymal stem cells by gene expression levels of fibroblasts cocultured with control inserts. Genes in the table demonstrated consistent and statistically significant fold changes (p < 0.05) in response to MSCs. All changes in mRNA levels were statistically significant and reproduced in three independent experiments
KO knock out, MSC mesenchymal stem cell, WT wild type