Table 1.
Characteristics of real-time RT PCR
| Gene symbol | GenBank accession No. | Forward primer/Reverse primer/Probe | Primer/probe size (bp) | Amplicon size (bp) |
|---|---|---|---|---|
| ACTB | AY141970.1 | 5’CGTCCACCGCAAATGCTT’3 5’TCTCGTTTTCTGCGCAAGTTAG’3 5’TAGGCGGACTGTTAGC’3 |
18 22 16 |
85 |
| LPAR1 | NM_174047.2 | 5’GTTCAACACAGGGCCCAATACT’3 5’AACCGTCAGGCTGGTGTCA’3 5’TGACTGTCAGCACGTGGCTCCTGC’3 |
22 19 24 |
85 |
| LPAR2 | NM_001192235.1 | 5’GTCTCTGAGCCTGCGGGTTA’3 5’TGACTTCAGAGCCCAATTCTTG’3 5’CATCTATGCAACTGGGAG’3 |
20 22 18 |
80 |
| LPAR3 | NM_001192741.2 | 5’TCAAGTAAAGCACGGGCAGAA’3 5’CGGGTGAGAACGCATTGTG’3 5’AGGACACGTGCATTTACAGA’3 |
21 19 20 |
90 |
| LPAR4 | NM_001098105.1 | 5’ATCACCAATCTGGCCCTCTCT’3 5’CAGTGGCGGTTGAAATTGTAAAA’3 5’CTCTTTGTCTGCACTCTA’3 |
21 23 18 |
80 |
| AX | NM_001080293.1 | 5’CCATTATTCCCACAAGTTCACAGTA’3 5’TGTCAAGCATGTCACCAAAGGT’3 5’TGCCACAAAAGTGACAAA’3 |
19 20 18 |
80 |
| PLA2 | NM_001075864.1 | 5’CCATTATTCCCACAAGTTCACAGTA’3 5’TGTCAAGCATGTCACCAAAGGT’3 5’TGCCACAAAAGTGACAAA’3 |
25 22 18 |
80 |
| TNFα | AC_000180.1 | 5’CCCTTCCACCCCCTTGTT’3 5’TGATGGCAGACAGGATGTTGA’3 5’CTCACCCACACCATCAGCCGCA’3 |
18 21 22 |
85 |
| TNFα R1 | U90937.1 | 5’AGAGACAGGACACCATCTGC’3 5’GCAAAGCCCGAAGACAATCA’3 5’ATGTCCAACCCGACCTTCAA’3 |
20 20 20 |
188 |
| TNFα R2 | NM_001040490.2 | 5’ATAGTGGGGAAAGGACAGGC’3 5’AGGAGTGGATTTGGAGGTGG’3 5’GGTACCCCAGTTAGCTCAGG’3 |
20 20 20 |
175 |
| FAS | NM_174662.2 | 5’GCTAAGTCGCTTCAGTCGTG’3 5’GGATTGCTGAGTCGGACATG’3 5’CTGGAGTGGGTTGCCATTTC’3 |
20 20 20 |
179 |
| FasL | NM_001098859.1 | 5’AGTACAAGAAGGGCAGCCTT’3 5’TCATCTTCCCCTCCATCAGC’3 5’GTCAGTCCTGCAACAACCAG’3 |
20 20 20 |
165 |
| BCL2 | U92434.1 | 5’TCTTTGAGTTCGGAGGGGTC’3 5’GGCCATACAGCTCCACAAAG’3 5’ATGACCGAGTACCTGAACCG’3 |
20 20 20 |
162 |
| BAX | U92569.1 | 5’AACATGGAGCTGCAGAGGAT’3 5’CCAATGTCCAGCCCATGATG’3 5’AGTGGCGGCTGAAATGTTTT’3 |
20 20 20 |
208 |
| CASP3 | NM_001077840.1 | 5’TCTAAGCCATGGTGAAGAAGGAAT’3 5’GTCCCCTCTGAAGAAACTTGCTAA’3 5’TTTTGGAACCAACGGACCCGTCAA’3 |
24 24 24 |
84 |
| CASP8 | DQ319070.1 | 5’CTACTGAGAGAAGAGGCCCG’3 5’TTGATGGGTTCCTGCTTCCT’3 5’CTCAAGTTCCTAAGCCGGGA’3 |
20 20 20 |
210 |
| B-glycan | NC_005113.4 | 5’TTCCAGGACGAACCCAACA’3 5’CCTCAACATAAACGTGTCCATTCT’3 5’CGTCACCTTCAACATGGAGCTGTACAACAC’3 |
19 24 30 |
86 |
| DRAK2 | AC_000159.1 | 5’TAACAGAGCAAATCGGACACAAG’3 5’ATTCCGCTTTGACGACTCGCTGCC’3 5’AGCAGCACGGTGTCCAAGA’3 |
22 24 19 |
79 |
| MCL1 | NM_001099206.1 | 5’CCAGGCTCGGACGGCTCGC’3 5’CTTTACCTTTGTTGGTCGGAGAA’3 5’TCTCCAGGGACTGCCGATAT’3 |
19 23 20 |
82 |
| ERβ | NM_001001443.1 | 5’GCTGTCAGGCCTGTCGACTT’3 5’ATGCGGTACCCACACCTTTCT’3 5’CTATGAGGTTGGAATGGT’3 |
20 21 18 |
85 |
| DICE1 | AY386968.1 | 5’ACCGCCTTCACTGCCTTCT’3 5’AGATGAAAGATTTGCTGTCGATAGATT’3 5’CAGAGCTCGGACGGCTCGC’3 |
19 27 19 |
85 |
| SOX2 | AC_000158.1 | 5’CACCTGCAAGTTTCCTGCTT’3 5’TGTAAAATGTGTAGCCGCCG’3 5’CAGCATATTGACTCCACCGC’3 |
20 20 20 |
202 |