Fig. 1.

Algorithm development. Validation of the semi-automated quantification algorithms is shown through a representative images of the detection of NeuN and pTDP-43 by IHC (blue and red denote algorithm recognition in the processed image), b log-transformed regressions comparing automatic counts to manual counts (NeuN ICC = 0.959; pTDP-43 ICC = 0.913), and c Bland-Altman plots of the log-transformed data to test mean bias (NeuN = −0.019; pTDP-43 = 0.055) and 95% limit of agreement (NeuN = −0.440 to 0.402; pTDP-43 = −0.435 to 0.544) between automatic and manual counts. Bar = 100 μL