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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: J Pathol. 2016 May 20;239(3):335–343. doi: 10.1002/path.4730

Figure 4.

Figure 4

Western blot showing overexpression of GLI1 protein in case 1 with the MALAT1-GLI1 fusion (lane 3). Case 2 without the fusion does not show GLI1 overexpression (lane 4). As a control, 293T cells were either untransfected (lane 1) or transfected with a full length GLI1 plasmid (lane 2) (A). Transcriptional activity of truncated GLI1 was tested using a dual-luciferase assay. 293T cells were transiently transfected with a luciferase reporter construct containing 11 copies of a GLI1 binding site together with a construct containing either full length GLI1 or only exons 6 to 12 of GLI1. Cells were lysed 48 hours after transfection. Results are represented as means of relative luciferase activity of 3 independent experiments performed in triplicate. Error bars indicate SEM; *p<0.05 (B).