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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: Dev Biol. 2017 Jul 14;429(1):105–117. doi: 10.1016/j.ydbio.2017.07.002

Fig. 4. Simultaneous N-myc and c-myc inactivation after lens vesicle stage.

Fig. 4

(A) Realtime RT-PCR analysis of c-myc mRNA content in N-mycCtrl and N-mycMlr10-Cre lenses at P0 using c-myc and Gapdh TaqMan probes (n = 3). (B-D) Representative pictures of H & E stained N-myc;c-mycCtrl, N-mycMlr10-Cre and N-myc;c-mycMlr10-Cre lens at E17.5. (E-F) Volume measurements of N-myc;c-mycCtrl, N-mycMlr10-Cre and N-myc;c-mycMlr10-Cre lens (P60, n = 4) and eyes (P0, n = 5 and adult, n = 4). (G-H) Representative pictures of N-myc;c-mycCtrl and N-myc;c-mycMlr10-Cre P0 eyes. (I-J) Representative pictures of pH3 immunostaining of N-myc;c-mycCtrl and N-myc;c-mycMlr10-Cre E12.5 lenses (arrows indicate pH3+ cells). (K-N) Proportion of pH3+ (K), PCNA+ (L), BrdU+ (M), and cleaved-caspase-3+ (N) lens epithelial cells in N-myc;c-mycCtrl and N-myc;c-mycMlr10-Cre in different developmental stages (n = 3). t-tests performed in A,K and N. One-way ANOVA with Tukey's posttest performed in E and F. Error bars indicate S.D.; *p < 0.05, *** p < 0.001, ****p < 0.0001. Scale bar: 100 µm.