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Autophagy induction by MWCNTs as indicated by LC3-II formation across cell lines. Cells were treated with MWCNTs with strong autophagy induction in HEK 293 (A) and U87 (B) cell lines and MWCNTs with various autophagy induction in HEK 293 (C) and U87 (D) cell lines. The concentration of MWCNT was 100 µg/mL. Cells were incubated with MWCNT for 24 h before protein extraction and Western blotting using an LC3B antibody. β-Actin was used as a loading control.
