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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: ACS Nano. 2014 Feb 25;8(3):2087–2099. doi: 10.1021/nn500376w

Figure 5.

Figure 5

MWCNT-COOH and MWCNT 41 induce cell autophagy. (A) Induced cell autophagy is assayed by immunofluorescence in U87 reporter cells. Representative images of MWCNT 100 µg/mL were shown. Medium and rapamycin (Rapa, treated for 4 h at 4 µM) were respectively used as negative and positive controls. The scale bar (top left) is for 20 µm. (B) Transmission electron microscope images show autophagosomes formation in treated HEK 293 cells; the inset images are the enlargement of a single autophagosome (broad black arrow indicates an autophagosome; narrow black arrow in the inset indicates MWCNTs in autophagosomes). The scale bar represents 500 nm.