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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: ACS Nano. 2014 Feb 25;8(3):2087–2099. doi: 10.1021/nn500376w

Figure 8.

Figure 8

Lack of MWCNT-induced cell apoptosis. (A) After incubation with MWCNT-COOH or MWCNT 41 (100 µg/mL, 24 h), HEK293 cells were treated with annexin V PE and 7-AAD and analyzed by flow cytometry. Cells treated with culture medium or paclitaxel (100 nm) for 24h were used as negative and positive controls. (B) Procaspase 3 and cleaved caspase 3 were analyzed to detect the activation of apoptosis signaling. Cells treated with staurosporine (1 µM) for 24 h were used as positive control. β-Actin was used as loading control.