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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Anal Chem. 2017 Jul 11;89(15):7841–7846. doi: 10.1021/acs.analchem.7b01784

Figure 1.

Figure 1

Schematic diagram for multiplexed HyCCAPP with toehold-mediated capture/release strategy. Capture oligonucleotides contain a 30-nucleotide sequence, complementary to target DNAs, and an 8 to 9-nucleotide toehold at the 3′ end. Complete sequences of all oligonucleotides are provided in the SI Tables S-1 and S-2. The 3′ end is biotinylated to enable solid-phase capture with streptavidin magnetic beads. Release oligonucleotides are single-stranded oligonucleotides 38 to 39 nucleotides in length whose sequence is completely complementary to their capture oligonucleotide counterparts. Since the toehold sequence does not hybridize with the targeted genomic sequence, the release oligonucleotides are thermodynamically favored to hybridize with the capture oligonucleotides when introduced, displacing the previously bound targets. Sequential addition of different release oligonucleotides permits the programmable and stepwise elution of captured chromatin regions.