Table 1.
Protein Data Summarya
| normal | stressed | |||||||
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| proteins in uncaptured lysate | 1014 | 1503 | ||||||
|
| ||||||||
| 25S rDNA | ARX1 | CTT1 | RPL30 | 25S rDNA | ARX1 | CTT1 | RPL30 | |
| total proteins identified | 639 | 657 | 635 | 650 | 517 | 667 | 530 | 509 |
| enriched proteins | 75 | 69 | 72 | 88 | 34 | 48 | 38 | 39 |
| enriched at locus | 24 | 17 | 11 | 26 | 13 | 25 | 22 | 18 |
| proteins with expected GO term keywords | ||||||||
| nucleus | 30 | 25 | 31 | 37 | 13 | 18 | 11 | 12 |
| nucleotide binding | 21 | 21 | 29 | 31 | 14 | 19 | 11 | 13 |
| DNA | 14 | 14 | 13 | 15 | 9 | 9 | 10 | 11 |
| nucleolus | 11 | 9 | 10 | 10 | 7 | 7 | 5 | 7 |
| rRNA | 10 | 9 | 9 | 10 | 5 | 7 | 7 | 8 |
| RNA polymerase | 7 | 9 | 9 | 9 | 5 | 6 | 7 | 9 |
| ribosome biogenesis | 5 | 3 | 5 | 6 | 2 | 4 | 0 | 1 |
| ribosomal export | 3 | 2 | 3 | 3 | 0 | 0 | 0 | 0 |
| ribosomal proteins | 5 | 5 | 6 | 4 | 2 | 3 | 2 | 2 |
| stress response | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 |
a
Bottom-up proteomics identified around 600 proteins from each capture sample. Delta-percentile-rank analysis revealed between 34 and 88 enriched proteins, between 11 and 26 of which were enriched at specific locus (SI Table S-5). Many of the enriched proteins have GO terms associated with DNA, nucleus, and other chromatin-related functions. Complete lists of all proteins identified are provided in Supporting Information Table S-6.