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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: Nature. 2017 Jan 25;542(7639):55–59. doi: 10.1038/nature21035

Figure 1. The αVβ6:pro-TGF-β1 complex.

Figure 1

(a) Crystal structure of the 1:2 complex. Ribbon cartoon with domains in different colors, metal ions as gold spheres and bowtie tails in monomers colored magenta and orange. (b) Superimposition of αVβ6 βI domains with moving regions in magenta (closed) and green (open). Spheres show metal ions and equivalent Cα atoms at end of α7-helix. RGD Asp sidechain is shown in cyan with red oxygens and its hydrogen bonds and metal coordination to βI are dashed. (c–e) The three major integrin conformational states. (f–h) Negative-stain EM of a αVβ6 headpiece: proTGF-β1 2:2 complex preparation showing class averages representing the isolated αVβ6 headpiece (g), the 1:2 complex (h), and the 2:2 complex (i). Scale bar is 50 Å. All averages are in Extended Data Fig. S1. (i) SAXS ab initio reconstruction of αVβ6 head:pro-TGF-β1 1:2 complex shown as transparent surface with the fit complex crystal structure.