Figure 2.

Analysis of cap expression of the OneBac 2.0 cell lines. (A) AAV Cap expression was analyzed by Western blot analysis of cell extracts harvested after rAAV production. Cell extracts from 293 cells were generated 72 hr posttransfection, and cell extracts from rep/cap-expressing Sf9 cells were prepared 72 hr postinfection. The VP proteins were detected with mAb B1. (B) Quantitative Western blots generated as in (A) for comparison of the relative ratios of VP1, VP2, and VP3 expression in 293 cells compared with Sf9 cells, respectively. Expression levels of VP3 were arbitrarily set to 10, and expression levels of VP2 and VP1 were calculated as percentages thereof. The results of three experiments are given as means and SD. Please note the logarithmic scale of the y axis.