Fig. 3. Encapsulation of hydrophobic guest molecules during nanoprecipitation of hyp-polydendrons with varying G₂ dendron–PEG₁₆ content. (A) Fluorimetric analysis of hyp-polydendron nanoprecipitates containing Nile Red produced from varying G₂ dendron–PEG₁₆ initiator ratios of 100 : 0 (solid black), 90 : 10 (red dash), 75 : 25 (blue dash), 50 : 50 (green dash), 27 : 75 (pink dash), 10 : 90 (grey dash) and 0 : 100 (cyan dash); (B) comparison of the observed I ₁/I ₃ ratio of pyrene within varying solvents (open red squares)^53,54 and encapsulated within hyp-polydendrons synthesized using different G₂ dendron–PEG₁₆ initiator ratios (open blue circles).