Fig. 4. Preliminary evaluation of oral dosing potential for hyp-polydendron nanoprecipitates with varying G₂ dendron–PEG₁₆ content. (A) Schematic representation of hyp-polydendron nanoprecipitates passing from (i) apical (top) compartment (modelling the gut side) of a differentiated Caco-2 cell monolayer through to the basolateral (bottom) compartment (modelling the blood side) to evaluate potential for intestinal epithelium permeation, and (ii) movement of hyp-polydendron nanoprecipitates from the basolateral to the apical side of the monolayer; (B) comparison of permeation of a hyp-polydendron (10 : 90 G₂ dendron–PEG₁₆ ratio) nanoprecipitate (blue squares) and aqueous/DMSO solution (<1% v/v) of Nile Red (red circles) through a Caco-2 monolayer – B > A permeation (open symbols); A > B permeation (closed symbols) (C) apparent permeability (P _app) of Nile Red when encapsulated in hyp-polydendron nanoprecipitates of varying G₂ dendron–PEG₁₆molar ratios (open green triangles) and as an aqueous/DMSO solution (<1% v/v) (red line).