Table 2. Effect of solution and dilution concentrations on hyp-polydendron nanoprecipitation with varying G2–PEG16 molar ratios.
G2–PEG16 molar ratio | Initial THF concentration (C I; mg mL–1) | Final H2O concentration (C F; mg mL–1) | D z a (nm) | PdI a | ζ (mV) |
100 : 0 | 10 | 2 | 106 | 0.083 | –38.0 |
100 : 0 | 10 | 0.1 | 134 | 0.064 | –34.3 |
100 : 0 | 5 | 1 | 81 | 0.083 | –38.2 |
100 : 0 | 5 | 0.05 | 93 | 0.071 | –20.4 |
90 : 10 | 10 | 2 | 173 | 0.076 | –30.4 |
90 : 10 | 10 | 0.1 | 205 | 0.087 | –33.1 |
90 : 10 | 5 | 1 | 116 | 0.069 | –25.9 |
90 : 10 | 5 | 0.05 | 110 | 0.087 | –17.6 |
75 : 25 | 10 | 2 | 155 | 0.085 | –25.6 |
75 : 25 | 10 | 0.1 | 190 | 0.097 | –39.0 |
75 : 25 | 5 | 1 | 110 | 0.073 | –26.5 |
75 : 25 | 5 | 0.05 | 135 | 0.092 | –30.2 |
50 : 50 | 10 | 2 | 148 | 0.061 | –29.3 |
50 : 50 | 10 | 0.1 | 150 | 0.073 | –29.6 |
50 : 50 | 5 | 1 | 115 | 0.067 | –28.2 |
50 : 50 | 5 | 0.05 | 104 | 0.072 | –20.7 |
25 : 75 | 10 | 2 | 121 | 0.072 | –28.7 |
25 : 75 | 10 | 0.1 | 110 | 0.104 | –24.0 |
25 : 75 | 5 | 1 | 93 | 0.078 | –30.4 |
25 : 75 | 5 | 0.05 | 77 | 0.140 | –16.5 |
10 : 90 | 10 | 2 | 129 | 0.058 | –31.3 |
10 : 90 | 10 | 0.1 | 108 | 0.074 | –23.2 |
10 : 90 | 5 | 1 | 94 | 0.091 | –29.9 |
10 : 90 | 5 | 0.05 | 78 | 0.090 | –28.9 |
0 : 100 | 10 | 2 | 121 | 0.074 | –39.8 |
0 : 100 | 10 | 0.1 | 116 | 0.072 | –30.0 |
0 : 100 | 5 | 1 | 90 | 0.083 | –39.7 |
0 : 100 | 5 | 0.05 | 89 | 0.092 | –27.8 |
aMeasured by dynamic light scattering.