Table 2. Relative crystal volume used for structure determination in recent macromolecular electron diffraction studies.

	PDB code	Detector	d (Å)	Space group	Unit-cell dimensions (Å)	No. of crystals	Individual crystal size (µm) and total diffracted volume†	No. of unit cells‡ (×106)	Relative unique diffracted intensity§ (×106)
Lysozyme	5o4w	Hybrid pixel	2.1	P21212	105 × 68 × 32	1	0.2 × 0.5 × 1.4 (0.14 µm3)	0.6	1.4
Lysozyme (Nannenga, Shi, Leslie et al., 2014 ▸)	3j6k	CMOS	2.5	P43212	76 × 76 × 37	1	0.5 × 2.0 × 2.0 (2 µm3)	9.4	18
Catalase (Nannenga, Shi, Hattne et al., 2014 ▸)	3j7b	CMOS	3.2	P212121	68 × 172 × 182	1	0.15 × 4.0 × 6.0 (3.6 µm3)	1.7	14
Catalase (Yonekura et al., 2015 ▸)	3j7u	CCD	3.2	P212121	69 × 173 × 206	58	0.1 × 2.0 × 2.0 (23 µm3)	9.4	77
Ca2+-ATPase (Yonekura et al., 2015 ▸)	3j7t	CCD	3.4	C2	166 × 64 × 147 (β = 98°)	99	0.1 × 2.0 × 2.0 (40 µm3)	25	490

^†The illuminated crystal size used for data acquisition is estimated from the reported crystal dimensions and the aperture sizes used; for the structures with PDB codes 3j7u and 3j7t (Yonekura et al., 2015 ▸) we assumed that the plate-like crystals had a surface area of 2 × 2 µm. The total diffracted volume (indicated by the number in parentheses) takes the number of crystals required for the three-dimensional data set into account.

^‡The required number of unit cells was calculated by dividing the total diffracted volume by the unit-cell volume.

^§We calculated the relative unique diffracted intensity by dividing the number of required unit cells (given in the previous column) by the number of asymmetric units in the unit cell and multiplying the result by the cube of the resolution of the data set.