Figure 2.

The effect of 30 minutes ischemia followed by different lengths of reperfusion (24, 48, or 72 hours) on renal injury, and the effect of late administration of the IκB kinase inhibitor IKK16 24 hours into reperfusion. Representative histological hematoxylin‐eosin images (scale bar, 100 μm) of rat renal tissue were taken from groups without renal ischemia (sham+vehicle) (A) or from rats subjected to 30 minutes of renal ischemia followed by reperfusion for 24 hours (B), 48 hours (+vehicle) (C), and 72 hours (+vehicle) (D), and after 48 hours of reperfusion with treatment of IKK16 (1 mg/kg IV) 24 hours into reperfusion (E). Ten randomly selected fields from 3 individual kidneys (n=3) per group were selected and analyzed (total fields=30) for the determination of percentage background white space using ImageJ software, representing tubular dilatation (F). Data are presented as mean±SEM for the number of observations. *P<0.05 vs pre‐ischemia.