Effect of YOYO-1 dye on DNA
digestion by λ exonuclease. The digested
molecules were analyzed using agarose gel electrophoresis. Different
YOYO-1 dye concentrations were tested in increasing order from lane
7 to lane 1 and are shown in molar ratio as YOYO-1:nt pair. M, λ/HindIII
digest (kb), 0.6, 2.0, 2.3, 4.4, 6.6, 9.4 and 23.1; S, λ DNA
marker, 48.5 kb; C, digested molecules in the absence of YOYO-1
dye.