Figure 5.

HDAC1‐depletion stimulates basic fibroblast growth factor (bFGF) expression in human CMCs. A, qPCR assays evaluating the expression of known trophic factors involved in cell‐mediated cardiac repair in shHDAC1, shNT, or untransduced CMCs. Values are mean±SEM (n=4). qPCR data were log base 10 (y=log₁₀ y) transformed and analyzed by 2‐way ANOVA. P values were calculated using the post hoc Bonferroni multiple comparison test. B, Representative immunoblot evaluating bFGF expression in total protein isolates derived from shHDAC1, shNT, or untransduced CMCs (n=4). C, Densitometric quantification of bFGF immunoblots (expression relative to β‐actin). Values are mean±SEM (n=4). Western blot data were log base 10 (y=log₁₀ y) transformed and analyzed by unpaired, 1‐way ANOVA. P values were calculated using the post hoc Bonferroni multiple comparison test. All experiments utilized 1:3 shRNA viral titer dilutions. HDAC1 indicates histone deacetylase 1; shHDAC1, short hairpin RNA‐histone deacetylase 1; shNT, short hairpin RNA‐non target; UT, untransduced.