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. 2017 Jul 19;6(7):e006349. doi: 10.1161/JAHA.117.006349

Figure 6.

Figure 6

Ataxia telangiectasia mutated (ATM) haplodeficiency suppresses post–myocardial infarction (MI) angiogenesis. A, Immunohistochemical staining and bar graph showing CD31 expression and angiogenesis at day 28 in the hearts of ATM +/+ and ATM +/− mice post MI. B, Western blot analysis (C) showed CD31, vascular endothelial cadherin (VE‐cadherin), and vascular endothelial growth factor (VEGF) expressions in the hearts of ATM +/+ and ATM +/− mice at day 7 post MI. D, Quantitative real‐time polymerase chain reaction analysis showed the mRNA levels of chemokine (C‐X‐C motif) ligand (Cxcl) 1, Cxcl12, VEGF, and Cxcl8 in isolated cardiac fibroblasts (CFs) from ATM +/+ and ATM +/− mice after MI surgery. E, Western blot analysis of VEGF expression in cultured medium of isolated CFs from ATM +/+ and ATM +/− mice after MI surgery. F, Representative images and bar graph showing tube formation capacity of human umbilical vein endothelial cells on matrigel with stimulation of conditional culture medium from isolated CFs of ATM +/+ and ATM +/− mice after MI surgery. n=5 in each group. *P<0.05 vs corresponding ATM +/+ group. Bar=50 μm. # P<0.01. IL indicates interleukin; NS, not significant.