Table 6.

Two‐sample t‐tests for diffusion coefficients from free subpopulations. t‐Values, degrees of freedom (df), P‐values, and levels of significance (LOS) from two‐sample t‐tests (α = 0.05) are listed. The samples of InlB₃₂₁ + nystatin, InlB₃₂₁ + Latrunculin A, and Fab + cytochalasin D were not normally distributed according to the Kolmogorov–Smirnov test (α = 0.1). LOS are classified as follows: P > 0.05 no significant difference between means (n.s.), P < 0.01 very significant difference (**), and P < 0.001 highly significant difference (***)

Sample 1	Sample 2	t	df	P	LOS
Fab	Fab + cytochalasin D	0.3	118	0.7	n.s.
	Fab + latrunculin A	2.7	118	0.007	**
	Fab + MCD	8.8	118	1E‐14	***
	Fab + nystatin	2.8	118	0.006	**
	Fab + colchicine	0.9	118	0.4	n.s.
	Fab at 32 °C	18.7	118	5E‐37	***
InlB321	InlB321 + cytochalasin D	4.4	115	3E‐05	***
	InlB321 + latrunculin A	5.7	115	1E‐07	***
	InlB321 + MCD	9.7	115	1E‐16	***
	InlB321 + nystatin	2.9	115	0.004	**
	InlB321 + colchicine	2.7	115	0.009	**
	InlB321 at 32 °C	23.3	115	2E‐45	***
Fab	InlB321	7.1	115	9E‐11	***
Fab + cytochalasin D	InlB321 + cytochalasin D	3.7	118	3E‐04	***
Fab + latrunculin A	InlB321 + latrunculin A	0.2	118	0.9	n.s.
Fab + MCD	InlB321 + MCD	5.6	118	2E‐07	***
Fab + nystatin	InlB321 + nystatin	3.2	118	0.002	**
Fab + colchicine	InlB321 + colchicine	9.6	118	2E‐16	***
Fab at 32 °C	InlB321 at 32 °C	8.2	118	4E‐13	***