Table 10.

Diffusion coefficients and amplitudes from the fits of cumulative occurrences. Results with respective SEMs are shown for one‐ and two‐population fits (Eqns (4) and (5)) of the mean cumulative occurrences of diffusion coefficients obtained by uPAINT at 23 and 32 °C (N = 60 cells). Amplitudes signify the relative proportion of particles whose motion can be described accurately by the respective diffusion coefficient

	One population		Two populations
	A1	D1/μm²·s−1	A1	D1/μm²·s−1	D2/μm²·s−1
Fab	1.00 ± 0.01	0.119 ± 0.005	0.94 ± 0.01	0.134 ± 0.004	4E‐4 ± 9E‐4
+ cytochalasin D	1.00 ± 0.01	0.119 ± 0.005	0.95 ± 0.01	0.131 ± 0.004	2E‐4 ± 1E‐3
+ latrunculin A	1.00 ± 0.01	0.143 ± 0.005	0.96 ± 0.01	0.153 ± 0.005	3E‐6 ± 0
+ MCD	1.00 ± 0.01	0.067 ± 0.003	0.92 ± 0.01	0.080 ± 0.001	5E‐4 ± 2E‐4
+ nystatin	0.98 ± 0.02	0.077 ± 0.008	0.79 ± 0.01	0.135 ± 0.002	0.001 ± 9E‐5
+ colchicine	1.00 ± 0.01	0.116 ± 0.005	0.94 ± 0.01	0.130 ± 0.004	2E‐4 ± 2E‐3
At 32 °C	1.01 ± 0.01	0.259 ± 0.011	0.97 ± 0.01	0.269 ± 0.011	1E‐5 ± 0
InlB321	0.98 ± 0.02	0.054 ± 0.004	0.81 ± 0.01	0.089 ± 0.001	0.0014 ± 8E‐5
+ cytochalasin D	0.99 ± 0.01	0.076 ± 0.004	0.87 ± 0.01	0.103 ± 0.001	0.0013 ± 7E‐5
+ latrunculin A	0.98 ± 0.01	0.091 ± 0.006	0.86 ± 0.01	0.130 ± 0.001	0.0016 ± 1E‐4
+ MCD	0.98 ± 0.01	0.036 ± 0.003	0.81 ± 0.01	0.058 ± 0.001	0.0013 ± 8E‐5
+ nystatin	0.97 ± 0.02	0.049 ± 0.005	0.76 ± 0.01	0.100 ± 0.001	0.0014 ± 8E‐5
+ colchicine	0.98 ± 0.02	0.053 ± 0.004	0.82 ± 0.01	0.084 ± 0.001	0.0014 ± 7E‐5
At 32 °C	1.00 ± 0.01	0.176 ± 0.008	0.92 ± 0.01	0.205 ± 0.004	0.0013 ± 4E‐4