Figure 6.

Binding of CRE to the LoxP recognition sequence. EMSAs with the unmethylated LoxP recognition sequence (lanes 1–4) and the fully methylated LoxP site (lanes 5–8) bound to recombinant CRE protein (lanes 2–4 and 6–8) or with probe alone (lanes 1 and 5). The retarded mobility shift complex is indicated by the black arrow to the left. In lanes 3 and 7, the retarded mobility shift complex has been competed with 200-fold molar excess of specific LoxP competitor probe, while in lanes 4 and 8, the retarded mobility shift complex has been competed with nonspecific (NS) competitor.