Table 2. Plasmids used in this study.

Plasmid	Description	Source or reference
pRS315	CEN, LEU2, vector	Sikorski and Hieter (1989)
pFR246	pRS315 Ypk1(S51A S71A T504A S644A T662A)-myc	This study
pFR249	pRS315 Ypk1(S51A T57A S71A T504A S644A T662A)-myc	This study
pFR252	pRS315 Ypk1(S51A T57A S71A T504A S644A S653A T662A)-myc	Muir et al. (2015)
pFR255	pRS315 Ypk1(S51A T57A S63A S64A S71A T504A S644A S653A T662A)-myc	This study
pFR264	pRS315 Ypk1(S51A T57A S63A S64A S71A T504A S644A S653A T662A S671A S672A)-myc	Roelants et al. (2011)
pJEN9	pRS315 Ypk1(S51A T57A S63A S64A S71A T504A S644A S653A T662A S671A S672A S678A)-myc	This study
pKL28	pFR264 A671S	This study
pKL29	pFR264 A672S	This study
BG1805	2 µm, URA3, PGAL1, C-terminal tandem affinity (TAP) tag vector	Open Biosystems/Dharmacon/GE Healthcare
pAX50	BG1805 Ypk1(L424A)-TAP	Muir et al. (2014)
pAM20	pRS315 Ypk1-myc	Roelants et al. (2011)
pFR221	pRS315 Ypk1(T662A)-myc	Roelants et al. (2011)
pFR284	pRS315 Ypk1(T662E)-myc	This study
pJEN3	pRS315 Ypk1(S653A S671A S672A S678A)-myc	This study
pJEN6	pRS315 Ypk1(S653E S671D S672E S678E)-myc	This study
pFR220	pRS315 Ypk1(S644A)-myc	This study
pFR253	pRS315 Ypk1(S653A)-myc	This study
pFR268	pRS315 Ypk1(S671A S672A)-myc	This study
pJEN8	pRS315 Ypk1(S678A)-myc	This study
pFR234	pRS315 Ypk1(D242A)-myc	This study
pKL7	pRS315 Ypk1(D242A T662A)-myc	This study
pJEN4	pRS315 Ypk1(D242A S653A S671A S672A S678A)-myc	This study
pPL215	p416 pMET25 Ypk1-3HA	Niles et al. (2012)
pJEN5	p416 pMET25 Ypk1(S653A S671A S672A S678A)-3HA	This study
pKL32	p416 pMET25 Ypk1(T662A)-3HA	This study
YEp352GAL	URA3-marked GAL promoter-containing 2 µm vector	Benton et al. (1994)
pFR111	YEp352GAL-Ypk1(T504A)-myc	Roelants et al. (2010)
pAM76	YEp352GAL-Ypk1-myc	Roelants et al. (2002)
pFR112	Yep352GAL-Ypk1-myc	This study
YEp351	LEU2-marked 2 µm vector	Hill et al. (1986)
pKL49	YEp351 Ypk1 (S653A S671A S672A S678A)-myc	This study