Figure 6.

Analysis of Greb1l function in CRISPR/Cas9 mutagenized F0 mouse embryos. (A) CRISPR/Cas9 strategy for introducing the L1793R mutation using an ssODN donor template. The guide sequence is colored red and the adjacent PAM sequence (AGG) is indicated in turquoise. Point mutations engineered into the donor are shown as lowercase. (B–D) Whole-mount images highlighting the observed exencephaly in two mutants (C and D) carrying KI alleles as compared to wild type (B). (E–G) Coronal sections of microCT data show unilateral and complete kidney agenesis in mutagenized F0 embryos. The position of kidneys is indicated by red arrowheads and yellow dotted circles. Bar, 2 mm.