Figure 8.

Transient receptor potential melastatin subtype 8 (TRPM8) activation by dietary menthol inhibits reactive oxygen species (ROS) production and vasoconstriction in Angiotensin II (Ang II)–induced hypertensive mice. A, Cytosolic ROS measured by dihydroethidium (DHE) and (B) mitochondrial ROS measured by MitoSOX Red in the aortas from wild‐type (WT) and Trpm8^−/− mice. Bar, 200 μm. n=6. C through F, Western blot analysis of RhoA activation, myosin phosphatase targeting subunit 1 (MYPT1), Ser⁶⁹⁵‐phosphorylated MYPT1 (p‐MYPT1), myosin light chain (MLC), and Ser¹⁹‐phosphorylated MLC (p‐MLC) in aortas. n=4. *P<0.05, **P<0.01 vs saline‐normal diet (ND); ^# P<0.05, ^## P<0.01 vs Ang II‐ND. Data are expressed as mean±SEM. Men indicates ND plus menthol supplementation (0.5%).