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. 2017 Aug 4;6(8):e005506. doi: 10.1161/JAHA.117.005506

Figure 6.

Figure 6

Body and organ weights partially normalize after prenatal rapamycin treatment until adulthood. A, Survival curves show death of some rapamycin‐treated pups within the first 12 days after birth but not thereafter. Consequently, postnatal survival after 70 days is significantly reduced in rapamycin‐ (n=55) vs vehicle‐treated (n=48) animals (P<0.0005). Survival curves include all possible genotypes and both sexes. B, Body weight (BW) and tibia length (TL) in 11‐week‐old adult mice after prenatal rapamycin treatment were reduced compared with vehicle‐treated animals, indicating incomplete postnatal catch‐up growth. C, Heart weight (HW) in adult rapamycin‐treated animals completely normalizes during postnatal life, resulting in normal HW/BW and HW/TL ratios. D, Slightly reduced left ventricular (LV) wall thickness, but no major morphological changes within the LV, interventricular septum (IVS) or right ventricle (RV), were observed after prenatal mTORC1 inhibition compared to vehicle‐treated animals (hematoxylin and eosin staining, scale bar=1 mm). E, Kidney weight (KW), but not liver (LW) or spleen weight (SW), was significantly reduced in rapamycin‐ vs vehicle‐treated 11‐week‐old mice. F, LW/BW, KW/BW and SW/BW ratios in adult mice displayed no significant differences between the treatment groups. B, C, E, and F, Vehicle n=7, rapamycin n=9. (*P<0.05, **P<0.01). mTORC1 indicates mechanistic target of rapamycin complex 1.