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. 2017 Aug 19;6(8):e006114. doi: 10.1161/JAHA.117.006114

Figure 5.

Figure 5

Loss of Sirtuin 3 (SIRT3) suppresses Pink/Parkin‐mediated mitophagy. A, Representative confocal images of autophagic flux stained with LC3B (red), MitoTracker Green, and 4′,6‐diamidino‐2‐phenylindole (blue). Scale, 200 μm. B, LC3‐II expression was detected by Western blotting with quantitative analysis in SIRT3‐short hairpin RNA (shRNA) or negative control cells. C, LC3‐II expression was detected by Western blotting with quantitative analysis in lentivirus‐mediated SIRT3 (LV‐SIRT3) or negative control infected cells. D, Representative Western blot analysis of Pink1 and parkin following immunoprecipitation with SIRT3 antibody. E, Representative Western blot and (F) quantitative analysis of acetylated Pink1 and acetylated Parkin in SIRT3‐shRNA or negative control cells. G, Representative Western blot and (H) quantitative analysis of acetylated Pink1 and acetylated Parkin in LV‐SIRT3 or negative control cells. Results are expressed as fold‐change over untreated vector‐infected cells. Data are presented as means±SEM. *P<0.05, **P<0.01 vs untreated vector‐infected cells; # P<0.05, ## P<0.01 vs angiotensin II (Ang II)–treated vector‐infected cells. CQ indicates chloroquine.