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. 2017 Aug 2;6(8):e006387. doi: 10.1161/JAHA.117.006387

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© 2017 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Neutrophils/macrophages in CCR5^−/− Treg‐treated stroke mice slow down in the ischemic areas as compared with those in wild type (WT) Treg‐treated stroke mice. A, Representative 2‐photon time lapse images of splenocyte (Sp), WT Treg, or CCR5^−/−Treg‐treated stroke animals. Color lines represent the routes of rhodamine 6G‐labeled neutrophils/macrophages. Images are representative of 3 to 5 animals/group. B, Representative images of neutrophil/macrophage routes after intravenous injection of rhodamine 6G. C, Quantification of the moving speed of neutrophils/macrophages during the 2‐photon imaging period. ***P≤0.001. CCR5 indicates C‐C chemokine receptor type 5; Treg, regulatory T cell.