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. Author manuscript; available in PMC: 2018 Jul 25.
Published in final edited form as: Cell Rep. 2017 Jul 25;20(4):895–908. doi: 10.1016/j.celrep.2017.06.082

Figure 5. G bodies containing PFKL form in hypoxic mammalian cells.

Figure 5

(A) Pfk2p homolog, PFKL, forms granules in HepG2 cells maintained in 1% oxygen for 24 h. (B) Time course of PFKL granule formation under hypoxic conditions. HepG2 cells were kept in 1% oxygen for indicated times (1, 3, 8, and 24 h). As a control, cells were cultured under normoxic conditions. (C) Formation of PFKL bodies is dependent on RNA. RNaseA treatment of cells maintained in 1% oxygen for 24 h decreases PFKL bodies compared to vehicle. (A–C) All cells were fixed and stained with anti-PFKL antibody to examine granule formation. All scale bars 10 μm. [See also Figure S8]

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