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Atherosclerosis, aortic sinus; ApoE−/− mouse infected with MNV-4. Increased atherosclerosis in MNV-4 infected mice. Cells within atherosclerotic lesions are predominantly macrophages and positive for MNV-4 RNA (purple staining) by ISH (Inset: higher magnification). a) Movat’s pentachrome. b) Immunohistochemistry for the macrophage marker, Mac-2 (brown signal). c) In situ hybridization (ISH) for MNV-4 (nuclear fast red counterstain).
