Table 2. Qualitative evaluation of the efficiency of DNA polymerases at the primer extension and amplification steps on 1 µg of genomic DNA of variable global SSB frequencies.
| Choice of DNA polymerase |
High-break frequency (>1/kb) + piperidine
treatment |
Low-break frequency (<1/kb) + piperidine
treatment |
High-break frequency (>1/kb)
w/o piperidine treatment |
Low-break frequency (<1/kb) w/o
piperidine treatment |
| Sequenase 2.0 in primer extension | +++ | ++ | ++ | + |
| Pfu exo– in primer extension | +++ | +++ | +++ | +++ |
| Pfu exo– in PCR amplification | ++ | ++ | ++ | +++ |
| Taq in PCR amplification | +++ | +++ | +++ | +++ |
+, good; ++, very good; +++, excellent.