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Structures of MSPs. The MSPs are labeled with different fluorophores (F, 6-FAM; H, HEX; and N, NED). The primers consist of sequence modules of ‘cnc’ and ‘tnnt’ nucleotides (where n is either a, c, g or t). The modules are arranged in a different order in every primer; therefore, the base sequence of the primers is different but their melting temperatures are identical. They independently hybridize with corresponding tags.
